Fig 1: Overexpression of IRF6 inhibits the invasion and migration of ccRCC cells. a Wound healing detected the cell migration ability. b Transwell detected the cell invasion ability. c Statistical chart of cell mobility. d Statistical chart of cell invasion. ***P < 0.001 vs shRNA-NC. #P < 0.05, ##P < 0.01 vs Oe-NC
Fig 2: KIF20A partially reverses the effects of IRF6 on the invasion and migration of ccRCC cells. a Wound healing detected the cell migration ability. b Transwell detected the cell invasion ability. **P < 0.01, ***P < 0.001 vs Oe-NC. #P < 0.05, ##P < 0.01, ###P < 0.001 vs Oe-IRF6 + Oe-NC
Fig 3: Overexpression of IRF6 inhibits the growth and metastasis of ccRCC in vivo. a Mice were weighed and photographed before treatment. b The weight and volume of tumor tissue. c RT-qPCR detected the expression of IRF6. d Western blot detected the expression of IRF6. e The expression of ki-67 in tumor tissues was detected by IHC
Fig 4: Expression of KIF20A after cell transfection. a RT-qPCR detected the expression of KIF20A after inhibition of IRF6 and KIF20A expression. b Western blot detected the expression of KIF20A after inhibition of IRF6 and KIF20A expression. *P < 0.05, ***P < 0.001 vs shRNA-NC. #P < 0.05, ###P < 0.001 vs shRNA-IRF6 + shRNA-NC. c RT-qPCR detected the expression of KIF20A after overexpression of IRF6 and KIF20A. d Western blot detected the expression of KIF20A after overexpression of IRF6 and KIF20A. ***P < 0.001 vs Oe-NC. ###P < 0.001 vs Oe-IRF6 + Oe-NC
Fig 5: Expression of IRF6 in ccRCC cancer cell lines. a RT-qPCR detected the expression of IRF6 in different ccRCC cancer cell lines. b Western blot detected the expression of IRF6 in different ccRCC cancer cell lines. *P < 0.05, ***P < 0.001 vs HK2. c The expression of IRF6 in cells was detected by RT-qPCR after cell transfection. d The expression of IRF6 in cells was detected by western blot after cell transfection. *P < 0.05, **P < 0.01, ***P < 0.001 vs shRNA-NC. ##P < 0.01, ###P < 0.001 vs Oe-NC
Supplier Page from Abcam for Anti-IRF6 antibody [OTI2A12]